Exmere

    Wide-pore silica

    Exsil 300

    The first Exsil product – wide-pore 300 Å silica for proteins, peptides and large molecules.

    300 Å100 m²/g3 – 10 µmWide pore
    Request quotation Talk to a silica specialist
    Exsil 300

    Pore size

    300 Å (± 10 Å)

    Surface area

    100 m²/g

    Pore volume

    0.78 mL/g

    Particle sizes

    3 – 10 µm

    Silica type

    Available on request

    Bonded phases

    12

    What is Exsil 300

    Exsil 300

    Exsil 300 was Exmere's first product, developed as an alternative to Vydac TP phases. It is a robust, spherical silica for separating biological macromolecules, with 300 Å pores, a pore volume of 0.78 mL/g and a surface area of 100 m²/g.

    Exmere introduced the first sub-5 µm wide-pore media in 1985, and Exsil 300 remains available in 3 µm.

    Why Exsil 300

    Why chromatographers choose Exsil 300.

    3 µm wide pore

    The first 300 Å media available in 3 µm, bringing fast analysis to biochemistry.

    Complete reversed-phase set

    ODS, C18M, Octyl and Butyl for peptides and proteins.

    HAAX for proteins and sugars

    High-affinity anion exchanger (epoxide + PEI). Tolerates 30 column volumes of 0.1 M NaOH and does not react with aldehydes or ketones.

    PAH phase

    Polymeric phase for polyaromatic hydrocarbon analysis.

    Typical applications

    Where Exsil 300 is used.

    Tryptic digests

    Fast tryptic digest analysis on 50 × 4.6 mm Exsil 300/3 C8.

    Proteins on HAAX

    Protein separations, e.g. egg-white proteins.

    Sugars on HAAX

    Sugar separations, compatible with acetone eluents (ELSD detection).

    Diphenyl comparison

    Exsil 300 Diphenyl, endcapped, compared with Vydac TP219.

    Performance evidence

    Performance evidence

    GPC calibration

    GPC calibration curves (Kd vs. log Mw) recorded for three Exsil 300 production batches.

    Technical characteristics

    Engineered properties.

    Particle sizes3, 5, 7, 10 µm
    Pore size300 Å (± 10 Å)
    Specific surface area100 m²/g
    Pore volume0.78 mL/g
    Silica typeAvailable on request
    Base silicaExsil 300 (also 340 Å silica)
    Carbon loadAvailable on request
    MorphologySpherical

    Available modifications

    Bonded phases on Exsil 300.

    Reversed phase

    • ODS (C18) · L1
    • C18M · L1
    • Octyl C8 · L7
    • C6 · L15
    • Butyl C4 · L26
    • Diphenyl
    • PAH (polymeric)

    Normal phase

    • Silica 300 Å · L3
    • Silica 340 Å · L3

    Ion exchange

    • HAAX (PEI)
    • PAX
    • SAX · L14
    Explore full bonded-phase portfolio

    Sizes & formats

    Sizes & formats

    Particle sizeScale
    3 µmFast analytical
    5 µmRoutine HPLC
    7 µmSemi-preparative
    10 µmPreparative

    FAQ

    Frequently asked questions.

    Next step

    Exsil 300 enquiry

    Request quotation Contact technical support