Exmere

    Silica family

    Exsil Mono

    The Next Generation of Silica

    Ultra high purity monosized silica bulk for HPLC, designed for exceptional efficiency, perfect reproducibility and scalable performance from UPLC to preparative chromatography.

    Monosized particlesHighly efficientPerfect reproducibilitypH stable to 11UPLC → Preparative
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    Scanning electron micrograph of monosized Exsil Mono 100 Si 5 µm silica particles
    SEM · Exsil Mono 100 Si · 5 µm

    Phase

    Si · C8 · C18

    Endcapping

    Yes

    Surface area

    350 m²/g · 100 m²/g

    Pore size

    100 Å · 300 Å

    Particle sizes

    1.7 – 10 µm

    Typical efficiency

    >90,000 pl/m (5 µm)

    Why Exsil Mono

    A new standard in HPLC packing media.

    Exmere Ltd. manufactures Exsil Mono from the silica particle to the finished bonded phase. This end-to-end process delivers high chromatographic performance at exceptional value — for column manufacturers, method developers and preparative labs that need a reproducible silica across every scale.

    • Tight control of silica synthesis
    • Controlled, reproducible bonding
    • Low variation in capacity factor and selectivity
    • Consistent column-to-column efficiency
    • Designed for reproducible methods

    Key benefits

    Engineered for performance and scale.

    Lower backpressure

    The narrow particle size distribution reduces column backpressure at analytical and preparative flow rates.

    Higher performance

    Monosized particles deliver consistently high plate counts and sharp peaks.

    Easy column packing

    Uniform particles pack reproducibly on analytical, semi-prep and DAC systems.

    UPLC to preparative

    Fully scalable — the same silica lineage from 1.7 µm UPLC through to 10 µm preparative.

    Perfect particle strength

    Robust particles designed for repeated preparative packing cycles under pressure.

    Unique bonding technology

    High and reproducible alkyl loading tightly controls capacity factor and selectivity.

    High loading capacity

    Very high alkyl loading supports preparative injections and high-load analytical work.

    pH stable up to 11

    Very high alkyl loading extends the operating pH range for basic mobile phases.

    Tailor-made modifications

    Custom bonded phases and particle specifications on request.

    Particle technology evidence

    What monosized silica looks like.

    Coulter counter particle size distribution and SEM micrograph for Exsil Mono 100 Si, 5 µm.

    Coulter counter particle size distribution of Exsil Mono 100 Si 5 µm — narrow monosized peak with D90/D10 = 1.07 and D50 = 4.78 µm

    Quality data

    Coulter counter particle size distribution

    A narrow, monosized peak around D50 = 4.78 µm with D90/D10 = 1.07 supports easy column packing, low backpressure and reproducible chromatographic performance.

    Scanning electron micrograph of Exsil Mono 100 Si 5 µm showing uniform spherical particles

    Microscopy

    SEM morphology

    Uniform spherical morphology under SEM — every particle contributes the same way to the packed bed.

    Full specification

    Engineered properties.

    PhaseSi, C8 and C18 (others on request)
    EndcappingYes
    Typical efficiency>90,000 plates/m (for 5 µm)
    Surface area350 m²/g and 100 m²/g
    Pore size100 Å and 300 Å
    Particle size1.7, 3, 5, 7 and 10 µm

    Full technical documentation, CoA and bonded-phase datasheets available on request.

    Batch-to-batch reproducibility

    Reproducible methods start with reproducible silica.

    Tightly controlled silica synthesis and bonding keep capacity factor and selectivity variations to a minimum — column to column, batch to batch.

    Chromatogram · Batch 1, 2 and 3

    Identical selectivity across three independent batches

    Overlaid chromatograms of Uracil, Phenylaceton, Benzene and Styrene on three independent batches of Exsil Mono 100 C18 5 µm, showing identical retention times and peak shapes

    Analytes

    1. 1Uracil
    2. 2Phenylaceton
    3. 3Benzene
    4. 4Styrene

    Overlaid separations on three independent batches of Exsil Mono 100 C18 5 µm show consistent retention and selectivity.

    Reference chromatogram — Exsil Mono brochure v1.7.

    Test conditions

    Column
    Exsil Mono 100 C18, 5 µm, 4.6 × 150 mm
    Mobile phase
    ACN / H₂O 60:40
    Flow rate
    1.0 mL/min
    Detector
    UV at 254 nm
    Injection
    3 µL

    Analytical comparison

    High efficiency with competitive backpressure.

    Side-by-side chromatograms from the brochure comparing Exsil Mono 100 C18 3 µm and a competitor 3.5 µm C18 column under matched conditions.

    Exsil Mono 100 C18, 3 µm

    Sharp peaks at competitive backpressure

    Chromatogram of Uracil, Phenol, N,N-Diethyl-M-Toluamid and Toluene on Exsil Mono 100 C18 3 µm — 172,461 plates/m at 60 bar

    Efficiency

    172,461 pl/m

    Backpressure

    60 bar

    Analytes

    1. 1Uracil
    2. 2Phenol
    3. 3N,N-Diethyl-M-Toluamid
    4. 4Toluene

    Reference chromatogram — Exsil Mono brochure v1.7.

    Competitor K 100 C18, 3.5 µm

    Reference column at matched conditions

    Chromatogram of Uracil, Phenol, N,N-Diethyl-M-Toluamid and Toluene on Competitor K 100 C18 3.5 µm — 170318 plates/m at 52 bar

    Efficiency

    170,318 pl/m

    Backpressure

    52 bar

    Analytes

    1. 1Uracil
    2. 2Phenol
    3. 3N,N-Diethyl-M-Toluamid
    4. 4Toluene

    Reference chromatogram — Exsil Mono brochure v1.7.

    Preparative

    Choose Exsil Mono for preparative separation.

    Exsil Mono is designed not only for analytical performance but also for scalable preparative use.

    Fully scalable from UPLC to preparative chromatography
    High loading capacity
    Easy, reproducible column packing
    Perfect particle strength for repeated packing cycles
    Available up to 250 kg per batch
    Suitable for DAC and MODcol Spring Column packing concepts

    Need preparative column packing support?

    Contact us for media selection, particle-size guidance and column packing advice — from gram-scale isolation through to production-scale DAC columns.

    Contact us

    DAC column packing

    Stable performance after repacking.

    MODcol Spring Column 1″ packed with Exsil Mono 100 C18 5 µm — no pressure increase and unchanged separation performance after 13 repacking cycles in the brochure example.

    Backpressure over 13 repacking cycles

    No pressure increase after 13 cycles

    Bar chart of column backpressure across 13 repacking cycles for Exsil Mono 100 C18 5 µm in a MODcol Spring Column showing stable pressure

    Backpressure recorded across 13 successive repacking cycles. Pressure remains stable, indicating robust particle strength.

    Exsil Mono brochure v1.7.

    Robustness

    13 cycles

    No pressure increase

    MODcol Spring Column 1″, Exsil Mono 100 C18, 5 µm.

    Chromatogram · 1st packing vs. 3rd repacking

    Same performance after repacking

    Two chromatograms comparing 1st packing and 3rd repacking of Exsil Mono 100 C18 5 µm, showing matching retention times and peak shapes

    Reference chromatogram — Exsil Mono brochure v1.7. Column packed by Dr. Maisch GmbH.

    Applications

    Designed for analytical, UPLC and preparative HPLC.

    Analytical HPLC

    High efficiency, reproducible selectivity and competitive performance for routine and method-development work.

    UPLC / UHPLC

    Small particle sizes down to 1.7 µm for high-resolution, high-throughput separations.

    Preparative HPLC

    Scalable media with high loading capacity and robust packing performance for gram- to kilogram-scale isolation.

    Available phases

    Available Exsil Mono phases.

    Exsil Mono Si

    Bare silica for normal-phase HPLC.

    Exsil Mono C8

    Endcapped octyl phase for reversed-phase methods.

    Exsil Mono C18

    Endcapped octadecyl phase — the workhorse for RP HPLC.

    Particle sizes

    1.7 · 3 · 5 · 7 · 10 µm

    Pore sizes

    100 Å · 300 Å

    Other bonded-phase modifications available on request. See the full bonded-phase portfolio.

    Next step

    Ready to build reproducible HPLC methods with Exsil Mono?

    Contact us for availability, technical guidance, bulk quantities and tailor-made modifications. A chromatography specialist will respond within one business day.

    Free technical evaluation samples

    Full technical documentation and CoA

    OEM & private-label supply

    Bulk quantities up to 250 kg

    Tailor-made bonded-phase modifications

    Response within one business day

    Exmere Ltd — HPLC Silica Engineering

    Unit 8, Boundary Way, Kellet Road Industrial Estate, Carnforth, Lancashire LA5 9XP, UK

    info@exmere.eu · +44 (0)1524 734451