Exmere

    PREPARATIVE AND PROCESS CHROMATOGRAPHY

    Engineered for reliable purification at scale.

    Exsil Pure 100 HS is a high-surface-area 100 Å stationary-phase platform developed for demanding preparative and process-scale separations — from laboratory evaluation to long-term commercial supply.

    10 µm Particle Size100 Å Pore SizeTonne-Scale Supply

    C18-2, C8, Phenyl-Hexyl, CN, PEI, Si, SAX, SCX · Manufactured by Exmere Ltd in the United Kingdom

    Figure H1

    SEM image — 10 µm spherical silica beads

    SEM micrograph of Exsil Pure 100 HS 10 µm spherical silica beads

    Scanning electron micrograph of 10 µm spherical silica beads used in Exsil Pure 100 HS preparative media.

    Product introduction

    A stationary phase developed for demanding process chromatography.

    Exsil Pure 100 HS combines a controlled high-surface-area silica structure with reproducible bonding chemistry and tightly managed manufacturing specifications. The product family is designed to deliver consistent selectivity, efficient mass transfer, reliable loading performance and predictable pressure behaviour across laboratory, pilot and production-scale applications.

    It provides a stable chromatographic platform for customers who require dependable performance, repeatable scale-up and secure long-term supply.

    Reliable Selectivity

    Consistent surface chemistry supports predictable method development and transfer.

    High Loading Performance

    The high-surface-area pore structure is designed for demanding preparative purification.

    Controlled Manufacturing

    Defined physical and chemical specifications support reproducible batch-to-batch performance.

    Industrial Scalability

    Available from evaluation quantities through pilot batches to long-term tonne-scale supply.

    Key technical properties

    A controlled physical foundation.

    The controlled combination of particle morphology, pore structure and surface chemistry provides the basis for reproducible chromatographic performance and reliable column packing.

    10 µm

    Nominal particle size

    D90 / D10 ≤ 1.6

    Particle-size distribution

    100 Å

    Pore diameter

    420 m²/g

    Specific surface area

    1.00 mL/g

    Pore volume

    Type B

    High-purity spherical silica

    Product family

    One silica platform. Multiple selectivities.

    Exsil Pure 100 HS is available in eight 10 µm chemistries covering reversed-phase, normal-phase, SFC and ion-exchange purification. Each phase provides a distinct chromatographic selectivity within the same high-surface-area silica platform.

    Primary

    Exsil Pure 100 HS C18-2

    High-surface-area C18 reversed-phase chemistry for preparative and process-scale purification. It provides strong hydrophobic retention and serves as the primary reversed-phase option within the Exsil Pure 100 HS portfolio.

    • Reversed-phase chromatography
    • Strong hydrophobic retention
    • Primary RP selectivity screening

    Exsil Pure 100 HS C8

    C8 reversed-phase chemistry with lower hydrophobic retention than C18-2 under comparable conditions. It provides complementary alkyl selectivity and earlier elution for strongly retained analytes.

    • Reversed-phase chromatography
    • Moderate hydrophobic retention
    • Reduced retention versus C18-2

    Exsil Pure 100 HS Phenyl-Hexyl

    Phenyl-hexyl reversed-phase chemistry combining hydrophobic retention with additional aromatic interaction potential. It provides complementary selectivity to C18 and C8, particularly for aromatic analytes.

    • Reversed-phase chromatography
    • Hydrophobic and aromatic interactions
    • Complementary aromatic selectivity

    Exsil Pure 100 HS CN

    Moderately polar cyano-bonded phase that can be operated in either reversed-phase or normal-phase mode. It provides complementary dual-mode selectivity for chromatographic method development.

    • Reversed-phase or normal-phase mode
    • Moderately polar cyano chemistry
    • Dual-mode selectivity screening
    Primary SFC

    Exsil Pure 100 HS PEI

    Polyethyleneimine-modified phase positioned as the primary SFC chemistry within the Exsil Pure 100 HS portfolio. It can also be used in normal-phase mode and is particularly suitable for highly basic analytes.

    • Primary SFC chemistry
    • Also suitable for normal phase
    • Selectivity for highly basic analytes

    Exsil Pure 100 HS Si

    Unbonded high-surface-area silica for normal-phase adsorption chromatography. Its polar silanol surface provides an orthogonal selectivity without a bonded ligand.

    • Normal-phase adsorption
    • Polar silanol interactions
    • Unbonded silica selectivity

    Exsil Pure 100 HS SAX

    Strong anion-exchange phase with quaternary ammonium functionality for the retention of anionic analytes.

    • Strong anion exchange
    • Quaternary ammonium functionality
    • Retention of anionic analytes

    Exsil Pure 100 HS SCX

    Strong cation-exchange phase with sulfonic acid functionality for the retention of cationic analytes.

    • Strong cation exchange
    • Sulfonic acid functionality
    • Retention of cationic analytes

    Batch-to-batch reproducibility

    Reproducibility across independent production batches.

    Reliable process chromatography depends on consistent performance across different production campaigns. Exsil Pure 100 HS is manufactured under controlled conditions to maintain tightly defined physical properties and chromatographic behaviour from batch to batch.

    Figure 6.1

    Lot-to-Lot Reproducibility — Overlay of 10 Lots

    Overlay chromatogram of 10 different lots of Exsil Pure 100 HS C18-2 showing near-identical retention and peak area

    Overlay of 10 independent lots of Exsil Pure 100 HS C18-2. Retention times 2.15, 3.22, 4.54 and 6.60 min. Peak-area and retention-time reproducibility across lots demonstrate the tight process control of the Exmere manufacturing platform.

    Test conditions

    Column
    250 × 20 mm, Exsil Pure 100 HS C18-2, 10 µm (NovoGROM, 2 µm frit)
    Mobile phase
    MeOH / H₂O 85:15
    Flow rate
    15 mL/min
    Detection
    UV @ 254 nm
    Temperature
    Ambient
    Pressure
    20 bar (max. 100 bar)
    pH range
    2.0 – 8.0

    Chromatographic reference comparison

    Designed for straightforward method evaluation.

    For customers working with established high-surface-area C18 methods, Exsil Pure 100 HS can be evaluated as an additional chromatographic supply option. Side-by-side testing under identical conditions demonstrates closely matched chromatographic selectivity compared with Luna® C18, 10 µm.

    Figure 7.1

    Chromatographic comparison under identical test conditions

    Overlay chromatogram: Exsil Pure 100 HS C18-2 (green) vs Luna® C18, 10 µm (blue) — four-peak test mix at 254 nm.
    Exsil Pure 100 HS C18-2, 10 µmLuna® C18, 10 µm

    Overlay of a four-component test mix on Exsil Pure 100 HS C18-2 (green) and Luna® C18, 10 µm (blue). Column 4.6 × 150 mm, 10 µm; mobile phase ACN / H₂O 60:40; flow 1.0 mL/min; injection 3 µL; detection UV 254 nm. Retention times and peak profiles are closely aligned across all four analytes under the evaluated conditions.

    These results demonstrate chromatographic comparability under the tested conditions. Suitability for an individual application should be confirmed using the customer's own method and acceptance criteria.

    Scale-up and supply

    From evaluation sample to tonne-scale supply.

    Exmere supports customers from initial chromatographic evaluation through pilot qualification and long-term commercial production.

    1. 1

      Evaluation Sample

    2. 2

      Laboratory Column

    3. 3

      Pilot Quantity

    4. 4

      DAC Qualification

    5. 5

      Production Batch

    6. 6

      Long-Term Tonne-Scale Supply

    Supply capabilities

    • Evaluation samples
    • Laboratory-scale quantities
    • Pilot-scale quantities
    • Commercial production batches
    • Tonne-scale manufacturing
    • Reserved production campaigns
    • Batch reservation
    • Customer-specific specifications
    • Reference and retain samples
    • Long-term supply agreements

    One stationary-phase platform from method evaluation to commercial production.

    Quality & qualification support

    Technical support for reliable qualification.

    Quality Documentation

    • Certificate of Analysis
    • Physical-property data
    • Chromatographic batch-release data
    • Batch-to-batch comparison
    • Reference samples
    • Retain samples
    • Traceable production records

    Customer Support

    • Evaluation samples
    • Method-transfer support
    • Loading studies
    • Scale-up support
    • DAC packing support
    • Customer-specific acceptance criteria
    • Confidentiality agreements
    • Long-term supply planning

    For strategic process customers, Exmere can establish dedicated specifications, qualification protocols and long-term supply arrangements.

    Evaluation sample request

    Evaluate Exsil Pure 100 HS in your own process.

    Request an evaluation sample and test Exsil Pure 100 HS under your existing chromatographic conditions.

    Contact & application

    Optional technical details

    All enquiries are treated confidentially. Data is used only to process your request and is not shared with third parties.

    Ready to evaluate Exsil Pure 100 HS?

    Start with an evaluation sample, a technical discussion or a confidential large-scale supply project.

    Luna® is a registered trademark of Phenomenex, Inc. Exmere Ltd is not affiliated with or endorsed by Phenomenex, Inc. Comparative references are provided solely for technical orientation. Product performance should be confirmed under the user's individual application conditions.