Exmere

    Exsil GFC family

    Exsil GFC Media

    High performance gel filtration chromatography phases for protein and polymer size separation.

    The Exsil GFC range is designed for robust, high efficiency size separation with high mechanical stability, low adsorption and broad applicability across proteins and synthetic polyelectrolytes.

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    The Exsil GFC family

    Four phases. One consistent GFC platform.

    Choose the pore size that matches your target molecular weight range. All phases share the same 5 µm particle size, high mechanical stability and low-adsorption surface chemistry.

    Exsil GFC 2000

    Coming soon

    Smaller-pore GFC phase for the lower MW segment.

    Pore size
    —
    Particle size
    5 µm
    Application range
    Coming soon
    Get notified

    Exsil GFC 3000

    Available

    For protein size separation in the lower to medium MW range.

    Pore size
    235 Å
    Particle size
    5 µm
    Application range
    15 K – 60 K
    View GFC 3000

    Exsil GFC 4000

    Available

    For high molecular weight proteins.

    Pore size
    400 Å
    Particle size
    5 µm
    Application range
    60 K – 500 K
    View GFC 4000

    Exsil GFC 5000

    Available

    For very high molecular weight proteins.

    Pore size
    800 Å
    Particle size
    5 µm
    Application range
    150 K – 1250 K
    View GFC 5000

    At a glance

    One GFC family. Three pore sizes. Broad molecular weight coverage.

    Together, the Exsil GFC 3000, 4000 and 5000 phases cover a broad molecular weight range for protein size separation.

    ProductPore sizeParticle sizeTypical application rangeMain application
    GFC 3000235 Å5 µm15 K – 60 KProteins / medium MW range
    GFC 4000400 Å5 µm60 K – 500 KHigh MW proteins
    GFC 5000800 Å5 µm150 K – 1250 KVery high MW proteins
    GFC 2000
    Coming soon
    		—5 µmLower MW segmentDetails to follow

    Key benefits

    Engineered for robust, reproducible GFC separations.

    High mechanical stability

    Can be packed at 7000 psi and operated at higher back pressures than typical soft SEC media.

    High efficiency

    Typically up to 100,000 plates/m for 5 µm particles.

    High protein recovery

    No evidence of protein loss for acidic or basic proteins tested.

    Low adsorption

    Extensively deactivated polar surface — suitable for basic proteins and charged polymers.

    Economical alternative

    High performance at a lower cost than established GFC ranges.

    Reproducible

    Manufactured by Exmere with the batch-to-batch consistency chromatographers expect.

    Applications

    Proteins and synthetic polyelectrolytes.

    The Exsil GFC range is used across two application domains: high efficiency protein sizing, and analysis of charged synthetic polymers that are notoriously difficult to chromatograph on conventional SEC media.

    Protein size separation

    Exsil GFC 3000, 4000 and 5000 cover the working range from around 15 K up to 1250 K molecular weight, with high efficiency, no evidence of protein loss and low adsorption of basic proteins.

    • • Robust high-throughput protein sizing at higher flow rates
    • • Standard 8 mm ID and higher-sensitivity 4.6 mm ID formats
    • • Resistant to routine high-pH clean-up cycles

    Synthetic polyelectrolytes

    Synthetic polyelectrolytes are challenging because charged polymers can interact strongly with the stationary phase. The extensively deactivated polar surface of the Exsil GFC range allows separation of polyanionic and polycationic polymers with minimal apparent interaction.

    • • Polyallylamine and poly(4-vinylpyridine) — polycationics
    • • Polyethyleneimines across a range of molecular weights
    • • Polyanionic polymers where supported by application data

    Polycationic

    Polyallylamine (17 kDa)

    Polyallylamine chromatograms on Exsil GFC 3000 and Exsil GFC 5000.

    Polyallylamine on Exsil GFC 3000 and Exsil GFC 5000. 250 × 4.6 mm, 1 mL/min, pH 4 100 mM ammonium formate, ELSD detection.

    Reference chromatogram — Exmere technical note on synthetic polyelectrolytes.

    Polycationic

    Poly(4-vinylpyridine) (50 kDa)

    Poly(4-vinylpyridine) at varying ionic strengths on Exsil GFC 5000.

    Poly(4-vinylpyridine) on Exsil GFC 5000. 250 × 4.6 mm, 1 mL/min, pH 4 ammonium formate at (A) 250 mM, (B) 100 mM, (C) 50 mM. ELSD detection.

    Reference chromatogram — Exmere technical note on synthetic polyelectrolytes.

    Polycationic

    Polyethyleneimines

    Polyethyleneimines on Exsil GFC 3000.

    Two low molecular weight PEIs (0.9 kDa and 1.5 kDa) and a higher MW PEI (25 kDa) on Exsil GFC 3000, 250 × 4.6 mm, 1 mL/min, pH 4 100 mM ammonium formate, ELSD detection.

    Reference chromatogram — Exmere technical note on synthetic polyelectrolytes.

    Looking for the right GFC phase for your application?

    Contact us for technical guidance, availability and quotations across the full Exsil GFC range.

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