Exmere

    Exsil GFC family · 800 Å

    Exsil GFC 5000

    High performance size separation of very high molecular weight proteins.

    800 Å5 µm150 K – 1250 KpH 2 – 7.57000 psi
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    Core positioning

    Completing the range for very high MW proteins.

    Exsil GFC 5000 complements the GFC 3000 and GFC 4000 ranges and provides a high efficiency product for very high molecular weight proteins with superior bed stability at an economical price.

    Technical specifications

    Product specifications.

    PhaseStable High Polarity
    Working pH rangepH 2 – 7.5
    Base silicaExsil 5000S, high purity silica
    Particle size5 µm
    Pore size800 Å
    Pore volume1.2 cc/g
    Cat. no.5000GU — Exsil GFC 5000, 5 µm
    Cat. no. (base silica)5000GSU — Exsil 5000S, 5 µm silica

    Key benefits

    Why Exsil GFC 5000.

    Exceptional stability

    Can be packed at 7000 psi.

    High efficiency

    Up to 100,000 plates/m for 8 mm ID.

    High recovery

    No evidence of protein loss expected — same bonding as GFC 3000/4000.

    Wide applicability

    Low adsorption of basic proteins.

    Cost effective

    High performance at low cost.

    Reproducible

    Typical Exmere reliability.

    1 · Size exclusion range

    Optimised for very high MW proteins.

    GFC of proteins on Exsil GFC 5000 shifts the retention of proteins to higher Kd values compared to Exsil GFC 3000 and GFC 4000. The optimum operating range is 150 K – 1250 K Daltons, significantly higher than the 60 K – 500 K range of GFC 4000.

    Together, the three phases (GFC 3000, 4000 and 5000) cover a broad molecular weight range for protein size separation from around 15 K up to 1250 K.

    2 · Protein separations

    Resolving Thyroglobulin and other very large proteins.

    The large pore size of Exsil GFC 5000 allows the resolution of the high molecular weight Thyroglobulin (MW 670,000), which is partially excluded on smaller pore media.

    Thyroglobulin · Riboflavin

    250 × 4.6 mm Exsil GFC 5000

    Chromatogram of Thyroglobulin and Riboflavin on 250 x 4.6 mm Exsil GFC 5000.

    Separation of Thyroglobulin and Riboflavin on 250 × 4.6 mm Exsil GFC 5000 at 1 mL/min using 50 mM potassium phosphate pH 7 / 150 mM sodium chloride eluent.

    Reference chromatogram — Exmere technical note.

    3 · High speed & high resolution

    Mechanical headroom for demanding workflows.

    Exsil GFC 5000 retains the excellent mechanical stability of the Exsil GFC 3000 phase and can be packed at 7000 psi and operated at up to at least 3000 psi. This extends the opportunity to carry out either high speed or high resolution separations to very high molecular weight proteins.

    The chromatograms show a high speed separation completed on a 250 mm column in 3 minutes and a high resolution separation using three 250 mm columns. In both cases the back pressure was in the region of 2000 – 2500 psi — well within the operating capabilities of Exsil GFC 5000, but around twice the maximum limit of some competitive materials.

    Thyroglobulin · Ovalbumin · Phenylalanine

    250 mm column (A) vs 750 mm column stack (B)

    High speed vs high resolution Exsil GFC 5000 separations.

    A) 250 × 4.6 mm Exsil GFC 5000 at 1 mL/min and B) 750 × 4.6 mm Exsil GFC 5000 at 0.33 mL/min. Eluent: 50 mM potassium phosphate pH 7 / 150 mM sodium chloride.

    Reference chromatogram — Exmere technical note.

    4 · Protein recovery & phase clean-up

    Same bonding, same expected robustness.

    Based on the same bonding as the Exsil GFC 3000 / 4000 series, similar high protein recovery combined with resistance to high pH cleaning can be expected on Exsil GFC 5000.

    Not sure which Exsil GFC phase you need?

    Compare the other members of the Exsil GFC family.

    Exsil GFC 2000

    Coming soon

    Smaller-pore GFC phase for the lower MW segment.

    Pore size
    —
    Particle size
    5 µm
    Application range
    Coming soon
    Get notified

    Exsil GFC 3000

    Available

    For protein size separation in the lower to medium MW range.

    Pore size
    235 Å
    Particle size
    5 µm
    Application range
    15 K – 60 K
    View GFC 3000

    Exsil GFC 4000

    Available

    For high molecular weight proteins.

    Pore size
    400 Å
    Particle size
    5 µm
    Application range
    60 K – 500 K
    View GFC 4000

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